Collagen Coated 6 Well Plate

Ready to use room temperature storage.

Collagen coated 6 well plate. Under the culture hood prepare a collagen coating solution 300 μg ml by combining the following into a 50 ml centrifuge tube or similar sterile container in order. We use 2 5mg ml collagen from rat tail sigma stock solution in 0 2 sterile acetic. To coat culture wells we dilute 20μl of collagen stock in 1ml of 30 ethanol per well 6 well plates and. Procedure for one 6 well plate or 12 well plate under the culture hood prepare 200 ml of 1 25x dpbs.

The grade of jellagen jellyfish collagen used to coat this. Flat bottom 5 pack 50 case 389 83 per case. Collagen is the most widely used extracellular matrix ecm protein for cell culture facilitating cell attachment growth differentiation migration and tissue morphogenesis. 266 µl of naoh 0 1 m.

Combine 5 6 ml of 10x pbs with 194 4 ml of 1x dpbs. In vitro use of collagen can exert effects on the adherence morphology growth migration and differentiation of a variety of cell types. We offer collagen i rat tail in liquid form and on precoated tissue culture plates and collagen i bovine in liquid form for 3d applications and as coating solution. Collagen i found in most tissues and organs is most plentiful in dermis tendon and bone.

Gibco collagen i rat tail is now available in pre coated tissue culture plates to help save time at the bench. 24 and 96 well cultureware plates coated with jellyfish collagen suitable for cell culture research purposes. Collagen coated tissue culture plates for improved results in promotion of adhesion growth differentiation.